This evening they visit Catalonia with Dybala now wearing the Number 10 jersey, while Gonzalo Higuain has a point to prove after being dropped from Leo Messi’s Argentina.
They kick off at 19.45 UK time (18.45 GMT).
Juve knocked the Blaugrana out of the quarter-final last season, winning 3-0 at home with a Paulo Dybala brace and holding out for a goalless draw at Camp Nou.
Join us for all the build-up and action as it happens from tonight’s Champions League clashes Barcelona-Juventus and Atletico Madrid-Roma.
Diego Simeone knows the city well from his time at their arch rivals Lazio and it’s an extremely tough group including Chelsea and Qarabag, so every point counts.
Eusebio Di Francesco and several other Roma players are making their all-time Champions League debuts, but host old hands Atletico Madrid.
The group phase of the tournament begins today with two Italo-Spanish showdowns.